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   현재위치 : HOME > Biology & Microbiology  >  Biotechnology  >  
Laboratory Manual for Biotechnology and Laboratory Science: The Basics
판매가격  : 40,000원
적립금  : 1,200점
출판사  : Benjamin Cummings
저자  : Lisa A. Seidman
발행일  : 08 Mar 2012
페이지 수  : 464면
ISBN  : 9780321644022
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Table of Contents INTRODUCTION I. SAFETY IN THE LABORATORY Unit Introduction SAFETY PART 1: CREATING A SAFE WORKPLACE Fundamental Principles Classroom Activity 1: Performing a Risk Assessment Classroom Activity 2: Exploring the CDC and OSHA Websites Classroom Activity 3: Responding to Emergencies SAFETY PART 2: WORKING SAFELY WITH CHEMICALSClassroom Activity 4: Understanding the Chemicals with which You Work Fundamental Principles Classroom Activity 5: Personal Protection Laboratory Exercise 1: Tracking the Spread of Chemical Contamination Classroom Activity 6: Analyzing Safety Issues in a Laboratory Procedure SAFETY PART 3: WORKING SAFELY WITH BIOLOGICAL HAZARDS Fundamental Principles Laboratory Exercise 2: Production of Bioaerosols and Factors Affecting Aerosol Production Unit Discussion Safety Contract, student copy Safety Contract, to turn in II. DOCUMENTATION IN THE LABORATORY Unit Introduction Classroom Activity 7: Be an Auditor Classroom Activity 8: Writing and Following an SOP Laboratory Exercise 3: Keeping a Laboratory Notebook Unit Discussion III. METROLOGY IN THE LABORATORY Unit Introduction Laboratory Exercise 4: Recording Measurements with the Correct Number of Significant Figures Classroom Activity 9: Constructing a Simple Balance Laboratory Exercise 5:Weight Measurements 1; Good Weighing Practices Laboratory Exercise 6: Weight Measurements 2; Performance Verification Laboratory Exercise 7: Volume Measurements 1; Proper Use of Volume Measuring Devices Laboratory Exercise 8: Volume Measurements 2; Performance Verification of a Micropipette Laboratory Exercise 9: Measuring pH with Accuracy and Precision Unit Discussion IV. SPECTROPHOTOMETRY Unit Introduction Laboratory Exercise 10: Color and the Absorbance of Light Laboratory Exercise 11: Concentration, Absorbance, and Transmittance Laboratory Exercise 12: Preparing a Standard Curve with Food Coloring and Using it for Quantitation Classroom Activity 10:Beer’s Law and Calculating an Absorptivity Constant Laboratory Exercise 13: Determination of the Absorptivity Constant for ONP Unit Discussion V. BIOLOGICAL SOLUTIONS Unit Introduction Classroom Activity 11: Getting Ready to Prepare Solutions with One Solute: Calculations Classroom Activity 12: Getting Ready to Prepare Solutions with One Solute: Ordering Chemicals Laboratory Exercise 14: Preparing Solutions with One Solute Laboratory Exercise 15: Preparing Solutions to the Correct Concentration Laboratory Exercise 16: Working with Buffers Laboratory Exercise 17: Preparing Breaking Buffer Laboratory Exercise 18: Preparing TE Buffer Laboratory Exercise 19: More Practice Making a Buffer Laboratory Exercise 20: Making a Quality Product in a Simulated Company Unit Discussion VI. ASSAYS Unit Introduction Laboratory Exercise 21: Two Qualitative Assays Laboratory Exercise 22:UV Spectrophotometry of DNA; Quantitative Applications Laboratory Exercise 23:UV Spectrophotometry of DNA, RNA, and Proteins; Qualitative Applications Laboratory Exercise 24: Using Spectrophotometry for Quality Control; Niacin Laboratory Exercise 25: A Quantitative Protein Assay; Learning the Assay Laboratory Exercise 26: Exploring Assay Verification Laboratory Exercise 27: The Beta-Galactosidase Enzyme Assay Laboratory Exercise 28: Comparing the Specific Activity of Two Preparations of Beta-Galactosidase Unit Discussion VII. SEPARATION METHODS Unit Introduction Classroom Activity 13: Separating Materials Using a Centrifuge, Planning Laboratory Exercise 29: Separation of Two Substances Based on Their Differential Affinities for Two Phases Laboratory Exercise 30: Separation of Dyes Using Paper Chromatography Laboratory Exercise 31: Separating Molecules by Agarose Gel Electrophoresis Laboratory Exercise 32: Using Agarose Gel Electrophoresis to Perform an Assay Laboratory Exercise 33: Optimizing Agarose Gel Electrophoresis Laboratory Exercise 34: Quantification of DNA by Agarose Gel Electrophoresis Unit Discussion VIII. GROWING CELLS Unit Introduction WORKING WITH BACTERIAL CELLS Laboratory Exercise 35: Aseptic Technique on an Open Lab Bench Laboratory Exercise 36: Working with Bacteria on an Agar Substrate; Isolating Individual Colonies Laboratory Exercise 37: Using a Compound Light Microscope Laboratory Exercise 38: Gram Staining Laboratory Exercise 39: Making Phosphate Buffered Saline Laboratory Exercise 40: The Aerobic Spread Plate Method of Enumerating Colony Forming Units Laboratory Exercise 41: Generation of an E. coli Growth Curve WORKING WITH MAMMALIAN CELLS Laboratory Exercise 42: Aseptic Technique in a Laminar Flow Hood Laboratory Exercise 43: Making Ham’s F12 Medium from Dehydrated Powder Laboratory Exercise 44: Examining, Photographing, and Feeding CHO (Chinese Hamster Ovary) Cells Laboratory Exercise 45: Counting Cells Using a Hemacytometer Laboratory Exercise 46: Subculturing CHO Cells Laboratory Exercise 47: Preparing a Growth Curve For CHO Cells Unit Discussion APPENDICES I Materials and Supplies that are Assumed to be Present in Your Laboratory II Calculating Standard Deviation II Periodic Table of Elements
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제품명 Laboratory Manual for Biotechnology and Laboratory Science: The Basics
판매가격 40,000원
제조사 Benjamin Cummings
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